Discovery of a Nitric Oxide Responsive Quorum Sensing Circuit in Vibrio cholerae.
Sajjad HossainIlana HecklerElizabeth M BoonPublished in: ACS chemical biology (2018)
Group behavior of the human pathogen Vibrio cholerae, including biofilm formation and virulence factor secretion, is mediated by a process known as quorum sensing. Quorum sensing is a way by which bacteria coordinate gene expression in response to population density through the production, secretion, and detection of small molecules called autoinducers. Four autoinducer-mediated receptor histidine kinases have been implicated in quorum sensing through the phosphotransfer protein LuxU: CqsS, LuxP/Q, CqsR, and VpsS (Vc1445). Of these receptor kinases, VpsS is predicted to be cytosolic, and its cognate autoinducer is currently unknown. In this study, we demonstrate that the nitric oxide-bound complex of a member of the recently discovered family of nitric oxide-responsive hemoproteins called NosP (VcNosP is encoded by Vc1444; this gene product is also known as VpsV) inhibits the autophosphorylation activity of VpsS and thus phosphate flow to LuxU. Therefore, we propose that VpsS contributes to the regulation of quorum sensing in a nitric-oxide-dependent manner through its interaction with NosP.
Keyphrases
- nitric oxide
- biofilm formation
- pseudomonas aeruginosa
- nitric oxide synthase
- gene expression
- candida albicans
- staphylococcus aureus
- hydrogen peroxide
- escherichia coli
- endothelial cells
- cancer therapy
- small molecule
- binding protein
- high throughput
- genome wide
- cystic fibrosis
- heat shock
- transcription factor
- induced pluripotent stem cells
- antimicrobial resistance
- single cell
- genome wide identification
- sensitive detection
- innate immune