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FFPE-Based NGS Approaches into Clinical Practice: The Limits of Glory from a Pathologist Viewpoint.

Filippo CappelloValentina AngerilliGiada MunariCarlotta CecconMarianna SabbadinFabio PagniNicola FuscoUmberto MalapelleAngelo Paolo Dei Tos
Published in: Journal of personalized medicine (2022)
The introduction of next-generation sequencing (NGS) in the molecular diagnostic armamentarium is deeply changing pathology practice and laboratory frameworks. NGS allows for the comprehensive molecular characterization of neoplasms, in order to provide the best treatment to oncologic patients. On the other hand, NGS raises technical issues and poses several challenges in terms of education, infrastructures and costs. The aim of this review is to give an overview of the main NGS sequencing platforms that can be used in current molecular diagnostics and gain insights into the clinical applications of NGS in precision oncology. Hence, we also focus on the preanalytical, analytical and interpretative issues raised by the incorporation of NGS in routine pathology diagnostics.
Keyphrases
  • clinical practice
  • healthcare
  • end stage renal disease
  • primary care
  • chronic kidney disease
  • newly diagnosed
  • palliative care
  • single cell
  • dna methylation
  • mass spectrometry
  • minimally invasive
  • replacement therapy