Contaminating reactivity of a monoclonal CCAAT/Enhancer Binding Protein β antibody in differentiating myoblasts.
Hamood AlSudaisNadine Wiper-BergeronPublished in: BMC research notes (2019)
We performed immunoprecipitation followed by mass spectrometry to identified myosin light chain 4 (MYL4) as the unknown band, suggesting that the Abcam monoclonal antibody directed against C/EBPβ is not pure, but contains a contaminating antibody against MYL4. Caution should be used when working in cells lines that express MYL4 to not confound the detection of MYL4 with that of C/EBPβ isoforms.
Keyphrases
- binding protein
- monoclonal antibody
- mass spectrometry
- induced apoptosis
- cell cycle arrest
- liquid chromatography
- capillary electrophoresis
- magnetic resonance imaging
- contrast enhanced
- computed tomography
- loop mediated isothermal amplification
- signaling pathway
- magnetic resonance
- high performance liquid chromatography
- pi k akt