Quantification of glucose-6-phosphate dehydrogenase activity by spectrophotometry: A systematic review and meta-analysis.
Daniel A PfefferBenedikt LeyRosalind E HowesPatrick AduMohammad Shafiul AlamPooja BansilYap BoumMarcelo BritoPimlak CharoenkwanArchie ClementsLiwang CuiZeshuai DengOchaka Julie EgesieFe Esperanza EspinoMichael E von FrickenMuzamil Mahdi Abdel HamidYongshu HeGisela HenriquesWasif Ali KhanNimol KhimSaorin KimMarcus Vinícius Guimarães de LacerdaChanthap LonAsrat Hailu MekuriaDidier MénardWuelton Marcelo MonteiroFrancois H NostenNwe Nwe OoSampa PalDuangdao PalasuwanSunil ParikhAyodhia Pitaloka PasaribuJeanne Rini PoespoprodjoDavid J PriceArantxa Roca-FeltrerMichelle E RohDavid L SaundersMichele D SpringInge SutantoKamala ThriemerThomas A WeppelmannLorenz von SeidleinAri Winasti SatyagrahaGermana BanconeGonzalo J DomingoRichard N PricePublished in: PLoS medicine (2020)
Our findings indicate that there is substantial variation in G6PD measurements by spectrophotometry between sites. This is likely due to variability in laboratory methods, with possible contribution of unmeasured population factors. While an assay-specific, universal quantitative threshold offers robust diagnosis at the 30% level, inter-study variability impedes performance of universal thresholds at the 70% level. Caution is advised in comparing findings based on absolute G6PD activity measurements across studies. Novel handheld quantitative G6PD diagnostics may allow greater standardisation in the future.