Flaxseed Ethanol Extracts' Antitumor, Antioxidant, and Anti-Inflammatory Potential.
Elisabeta Ioana CheraRaluca-Maria PopMarcel PârvuOlga SorițăuAna UifăleanFlorinela Adriana CătoiAndra CecanAndrada Gabriela NegoescuPatriciu Achimaș-CadariuAlina Elena PârvuPublished in: Antioxidants (Basel, Switzerland) (2022)
The antitumoral, antioxidant, and anti-inflammatory effects of flaxseed ethanol extract was screened. Phytochemical analysis was performed by measuring the total phenolic content and by HPLC-DAD-ESI MS. In vitro antiproliferative activity was appreciated by MMT test of four adenocarcinomas and two normal cell lines. In vitro, antioxidant activity was evaluated by DPPH, FRAP, H 2 O 2 , and NO scavenging tests. The in vivo growth inhibitory activity against Ehrlich ascites carcinoma (EAC) in female BALB/c mice was determined using the trypan blue test. In EAC mice serum and ascites total oxidative status, total antioxidant reactivity, oxidative stress index, malondialdehyde, total thiols, total nitrites, 3-nitrotyrosine, and NFkB were measured. The phytochemical analysis found an significant content of phenols, with lignans having the highest concentration. The extract had an significant in vitro antioxidant effect and different inhibitory effects on different cell lines. After treatment of EAC mice with flaxseeds extract, body weight, ascites volume and viable tumour cell count, serum and ascites oxidative stress, and inflammatory markers decreased significantly. The ethanol flaxseeds extract has potential antiproliferative activity against some ovary and endometrial malignant cells and EAC. This effect can be attributed to the phenols content, and its antioxidant and anti-inflammatory activity.
Keyphrases
- anti inflammatory
- oxidative stress
- ms ms
- induced apoptosis
- body weight
- cell free
- diabetic rats
- dna damage
- high fat diet induced
- ischemia reperfusion injury
- simultaneous determination
- mass spectrometry
- single cell
- multiple sclerosis
- endoplasmic reticulum stress
- cell therapy
- cell cycle arrest
- cell death
- insulin resistance
- solid phase extraction
- squamous cell
- signaling pathway