Comparison of Gelatin/Polylysine- and Silk Fibroin/SDF-1α-Coated Mesenchymal Stem cell-Seeded Intracranial Stents.

Endothelialization of the aneurysmal neck is essential for aneurysm healing after endovascular treatment. Mesenchymal stem cell (MSC)-seeded stents can promote aneurysm repair. investigated the biological effects of coated and uncoated nitinol intracranial stents seeded with MSCs on vascular cell and macrophage proliferation and inflammation. Two stent coatings that exert pro-aggregation effects on MSCs via different mechanisms examined: gelatin/polylysine (G/PLL), which enhances cell adhesion, and silk fibroin/SDF-1α (SF/SDF-1α), which enhances chemotaxis. aim to explore the feasibility of MSC-seeded coated stents in the treatment of intracranial aneurysms. The G/PLL coating provided the highest cytocompatibility and blood compatibility substrate for MSCs and vascular cells and promoted cell adhesion and proliferation. Moreover, it enhanced MSC secretion and regulation of vascular cell and macrophage proliferation and chemotaxis. Although the SF/SDF-1α coating promoted MSC secretion and vascular cell chemotaxis, it induced a greater degree of macrophage proliferation, chemotaxis, and secretion of pro-inflammatory factors. MSC-seeded stents coated with G/PLL may benefit stent surface endothelialization and reduce the inflammatory response after endovascular treatment of intracranial aneurysm. These effects may improve aneurysm healing and increase the cure rate. This article is protected by copyright. All rights reserved.