Bone infection poses a major clinical challenge that can hinder patient recovery and exacerbate postoperative complications. This study has developed a bioactive composite scaffold through the co-assembly and intrafibrillar mineralization of collagen fibrils and zinc oxide (ZnO) nanowires (IMC/ZnO). The IMC/ZnO exhibits bone-like hierarchical structures and enhances capabilities for osteogenesis, antibacterial activity, and bacteria-infected bone healing. During co-cultivation with human bone marrow mesenchymal stem cells (BMMSCs), the IMC/ZnO improves BMMSC adhesion, proliferation, and osteogenic differentiation even under inflammatory conditions. Moreover, it suppresses the activity of Gram-negative Porphyromonas gingivalis and Gram-positive Streptococcus mutans by releasing zinc ions within the acidic infectious microenvironment. In vivo, the IMC/ZnO enables near-complete healing of infected bone defects within the intricate oral bacterial milieu, which is attributed to IMC/ZnO orchestrating M2 macrophage polarization, and fostering an osteogenic and anti-inflammatory microenvironment. Overall, these findings demonstrate the promise of the bioactive scaffold IMC/ZnO for treating bacteria-infected bone defects.
Keyphrases
- bone regeneration
- room temperature
- quantum dots
- reduced graphene oxide
- gram negative
- bone mineral density
- tissue engineering
- visible light
- ionic liquid
- mesenchymal stem cells
- multidrug resistant
- stem cells
- soft tissue
- biofilm formation
- anti inflammatory
- bone loss
- oxide nanoparticles
- signaling pathway
- light emitting
- bone marrow
- candida albicans
- endothelial cells
- cystic fibrosis
- machine learning
- high resolution
- oxidative stress
- artificial intelligence
- cell adhesion
- induced pluripotent stem cells