The accuracy of haemoglobin A 2 measurements in the presence and absence of haemoglobin S.
Jane K MyburghRichard M SzydloBarbaraJ J BainPublished in: EJHaem (2023)
The quantification of haemoglobin A 2 by high-performance liquid chromatography (HPLC) was compared with quantification by capillary electrophoresis for control subjects and patients with sickle cell trait or sickle cell anaemia. Significant differences were found, with estimated values being higher by HPLC for control subjects and higher by capillary electrophoresis for sickle cell trait and sickle cell anaemia patients. There is an ongoing need for improved standardisation and alignment of methods.
Keyphrases
- capillary electrophoresis
- high performance liquid chromatography
- mass spectrometry
- simultaneous determination
- tandem mass spectrometry
- solid phase extraction
- liquid chromatography
- ms ms
- end stage renal disease
- chronic kidney disease
- newly diagnosed
- ejection fraction
- high resolution
- gene expression
- peritoneal dialysis
- iron deficiency
- patient reported