Optimization of the detection of inhibitory autoantibodies against the VWF-cleaving protease ADAMTS13 with an automated chemiluminescent ADAMTS13 activity immunoassay.

Marc G JacqueminIsa Van HorenbeeckMirjam DebasseJelle ToelenJoke SchoetersIngrid VanlinthoutKathelijne PeerlinckDaan DierickxChristine Van Laer

Published in: International journal of laboratory hematology (2020)

Incubation of plasma samples with normal plasma at 25°C, 37°C, or 37°C after addition of Ca2+ can be used in a Bethesda assay for quantifying the inhibitory activity of antibodies interfering with ADAMTS13 in the chemiluminescent HemosILR AcuStar ADAMTS13 Activity assay.

Keyphrases

high throughput
systemic lupus erythematosus
label free
sensitive detection
loop mediated isothermal amplification
fine needle aspiration
protein kinase