Dual colorimetric and fluorogenic probes for visualizing tyrosine phosphatase activity.

Suvendu BiswasBrandon S McCulloughElena S MaDollie LaJoieColin W RussellD Garrett BrownJune L RoundKatharine S UllmanMatthew A MulveyAmy M Barrios

Published in: Chemical communications (Cambridge, England) (2018)

Two resorufin-based substrates for protein tyrosine phosphatase (PTP) activity have been synthesized. These substrates provide sensitive fluorogenic readouts of PTP activity in vitro and in living cells at both acidic and neutral pH. In addition, the presence of the pathogenic bacteria Staphylococcus aureus was detected visually using a colorimetric readout.

Keyphrases

living cells
fluorescent probe
staphylococcus aureus
single molecule
gold nanoparticles
hydrogen peroxide
sensitive detection
small molecule
ionic liquid
protein kinase
protein protein
binding protein