The trajectory of vesicular proteomic signatures from HBV-HCC by chitosan-magnetic bead-based separation and DIA-proteomic analysis.
Lin CaoYue ZhouShuai LinChunyan YangZixuan GuanXiaofan LiShujie YangTong GaoJiazhen ZhaoNing FanYanan SongDongmin LiXiang LiZhuo LiFeng GuanZengqi TanPublished in: Journal of extracellular vesicles (2024)
Hepatocellular carcinoma (HCC) is a prevalent primary liver cancer often associated with chronic hepatitis B virus infection (CHB) and liver cirrhosis (LC), underscoring the critical need for biomarker discovery to improve patient outcomes. Emerging as a promising avenue for biomarker development, proteomic technology leveraging liquid biopsy from small extracellular vesicles (sEV) offers new insights. Here, we evaluated various methods for sEV isolation and identified polysaccharide chitosan (CS) as an optimal approach. Subsequently, we employed optimized CS-based magnetic beads (Mag-CS) for sEV separation from serum samples of healthy controls, CHB, LC, and HBV-HCC patients. Leveraging data-independent acquisition mass spectrometry coupled with machine learning, we uncovered potential vesicular protein biomarker signatures (KNG1, F11, KLKB1, CAPNS1, CDH1, CPN2, NME2) capable of distinguishing HBV-HCC from CHB, LC, and non-HCC conditions. Collectively, our findings highlight the utility of Mag-CS-based sEV isolation for identifying early detection biomarkers in HBV-HCC.
Keyphrases
- hepatitis b virus
- liquid chromatography
- mass spectrometry
- liver failure
- machine learning
- drug delivery
- simultaneous determination
- end stage renal disease
- ejection fraction
- newly diagnosed
- high resolution mass spectrometry
- small molecule
- big data
- chronic kidney disease
- molecularly imprinted
- tandem mass spectrometry
- peritoneal dialysis
- artificial intelligence
- solid phase extraction
- gene expression
- high resolution
- prognostic factors
- hyaluronic acid
- ultrasound guided
- high performance liquid chromatography
- wound healing
- risk assessment
- drug induced
- protein protein
- ms ms
- capillary electrophoresis
- patient reported
- human health