Distinct dual enzyme-like activities of Fe-N-C single-atom nanozymes enable discriminative detection of cellular glutathione.
Chunqi CaiChengyang ZhuLingling LvPengcheng HuangJunjie MaoFang-Ying WuKe-Yu DengPublished in: Chemical communications (Cambridge, England) (2023)
Fe-N-C single-atom nanozymes readily achieved discriminative detection of glutathione (GSH) over other biothiols with similar structure due to the difference between POD-like and OXD-like activities regarding the kind of reactive oxygen species. This colorimetric sensor demonstrated the heterogeneity of GSH levels in different cells and accurately monitored cellular GSH fluctuation.
Keyphrases
- fluorescent probe
- living cells
- reactive oxygen species
- loop mediated isothermal amplification
- induced apoptosis
- label free
- molecular dynamics
- real time pcr
- gold nanoparticles
- cell cycle arrest
- single cell
- aqueous solution
- sensitive detection
- metal organic framework
- oxidative stress
- hydrogen peroxide
- cell death
- cell proliferation