Clinical Assessment and Validation of a Rapid and Sensitive SARS-CoV-2 Test Using Reverse Transcription Loop-Mediated Isothermal Amplification Without the Need for RNA Extraction.

Melis N AnahtarGraham E G McGrathBrian A RabeNathan A TannerBenjamin A WhiteJochen K M LennerzJohn A BrandaConstance L CepkoEric S Rosenberg

Published in: Open forum infectious diseases (2020)

By incorporating a fast and inexpensive sample preparation step, RT-LAMP accurately detects SARS-CoV-2 with limited equipment for about US$6 per sample, making this a potentially ideal assay to increase testing capacity, especially in resource-limited settings.

Keyphrases

loop mediated isothermal amplification
sars cov
sensitive detection
respiratory syndrome coronavirus
high throughput
transcription factor
molecularly imprinted
high resolution
mass spectrometry