A Mechanoelastic Glimpse on Hyaluronan-Coated Extracellular Vesicles.
Debashish PaulAnirban PaulDipanjan MukherjeeSaroj SarojManorama GhosalSuchetan PalDulal SenapatiJaydeb ChakrabartiSamir Kumar PalTatini RakshitPublished in: The journal of physical chemistry letters (2022)
Cancer cells secrete extracellular vesicles (EVs) covered with a carbohydrate polymer, hyaluronan (HA), linked to tumor malignancy. Herein, we have unravelled the contour lengths of HA on a single cancer cell-derived EV surface using single-molecule force spectroscopy (SMFS), which divulges the presence of low molecular weight HA (LMW-HA < 200 kDa). We also discovered that these LMW-HA-EVs are significantly more elastic than the normal cell-derived EVs. This intrinsic elasticity of cancer EVs could be directly allied to the LMW-HA abundance and associated labile water network on EV surface as revealed by correlative SMFS, hydration dynamics with fluorescence spectroscopy, and molecular dynamics simulations. This method emerges as a molecular biosensor of the cancer microenvironment.
Keyphrases
- single molecule
- papillary thyroid
- molecular dynamics simulations
- squamous cell
- atomic force microscopy
- living cells
- high resolution
- squamous cell carcinoma
- gold nanoparticles
- lymph node metastasis
- molecular docking
- sensitive detection
- microbial community
- young adults
- heat shock protein
- quantum dots
- label free
- anaerobic digestion