Justification of direct Sanger sequencing application for detection of KIT and PDGFRα gene mutations in formalin-fixed, paraffin-embedded samples from gastrointestinal stromal tumours.
Katarzyna KiwerskaJoanna WroblewskaApolonia KaluznaAndrzej MarszalekPublished in: Journal of clinical pathology (2019)
Our results show that in GIST samples, carrying a broad spectrum of deletions, Sanger sequencing is a better, more sensitive method for mutational analysis of KIT and PDGFRα genes.