Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus.
Jianqiang ZhangCharles NfonChuan-Fu TsaiChien-Hsien LeeLindsay FredericksQi ChenAvanti SinhaSarah BadeKaren HarmonPablo PiñeyroPhillip GaugerYun-Long TsaiHwa-Tang Thomas WangPei-Yu Alison LeePublished in: BMC veterinary research (2019)
The SVV rRT-PCR and RT-iiPCR developed in this study are very sensitive and specific and have comparable diagnostic performances for SVV RNA detection. The SVV rRT-PCR can be adopted for SVV detection in laboratories. The SVV RT-iiPCR in a simple field-deployable system could serve as a tool to help diagnose vesicular diseases in swine at points of need.