Correlative montage parallel array cryo-tomography for in situ structural cell biology.
Jie E YangMatthew R LarsonBryan S SibertJoseph Y KimDaniel ParrellJuan C SanchezVictoria PappasAnil KumarKai CaiKeith ThompsonElizabeth R WrightPublished in: Nature methods (2023)
Imaging large fields of view while preserving high-resolution structural information remains a challenge in low-dose cryo-electron tomography. Here we present robust tools for montage parallel array cryo-tomography (MPACT) tailored for vitrified specimens. The combination of correlative cryo-fluorescence microscopy, focused-ion-beam milling, substrate micropatterning, and MPACT supports studies that contextually define the three-dimensional architecture of cells. To further extend the flexibility of MPACT, tilt series may be processed in their entirety or as individual tiles suitable for sub-tomogram averaging, enabling efficient data processing and analysis.
Keyphrases
- electron microscopy
- high resolution
- low dose
- mass spectrometry
- induced apoptosis
- high throughput
- high speed
- single molecule
- cell cycle arrest
- single cell
- high dose
- healthcare
- cell therapy
- big data
- electronic health record
- stem cells
- health information
- fluorescence imaging
- tandem mass spectrometry
- cell death
- high density
- smoking cessation
- signaling pathway
- social media
- liquid chromatography