Identification of the anabolic steroid 6β-chlorotestosterone in a dietary supplement.
Travis M FalconerRick A FlurerMary B JonesLisa M LorenzSarah E VoelkerPublished in: Drug testing and analysis (2018)
Capsules that were labeled to be performance-enhancing dietary supplements obtained during an investigation were found to contain an unrecognized steroid-like substance. This compound was isolated by liquid chromatography (LC) fraction collection and characterized using several qualitative analytical techniques, including ultraviolet (UV) spectroscopy, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-high resolution accurate mass-mass spectrometry (LC-HRAM-MS), as well as 1 H, 13 C, and two-dimensional nuclear magnetic resonance (NMR) spectrometry. This multi-technique analytical approach was used to identify the designer steroid as 6β-chloro-4-androsten-17β-ol-3-one (6β-chlorotestosterone), an analog of testosterone about which little has been published.
Keyphrases
- solid state
- liquid chromatography
- mass spectrometry
- high resolution
- gas chromatography mass spectrometry
- gas chromatography
- solid phase extraction
- tandem mass spectrometry
- high resolution mass spectrometry
- simultaneous determination
- magnetic resonance
- high performance liquid chromatography
- capillary electrophoresis
- high speed
- ms ms
- systematic review
- magnetic resonance imaging
- pet ct
- smoking cessation
- replacement therapy