Mobile-CRISPRi protocol optimization for Vibrionaceae .

Logan J GeymanMadeline P TannerNatalia Rosario-MeléndezJason M PetersMark J Mandel Julia C van Kessel

Published in: Microbiology resource announcements (2024)

Mobile clustered regularly interspaced palindromic repeats interference (Mobile-CRISPRi) is an established method for bacterial gene expression knockdown. The deactivated Cas9 protein and guide RNA are isopropyl β-D-1-thiogalactopyranoside inducible, and all components are integrated into the chromosome via Tn 7 transposition. Here, we optimized methods specific for applying Mobile-CRISPRi in multiple Vibrio species.

Keyphrases

gene expression
dna methylation
escherichia coli
pseudomonas aeruginosa
cystic fibrosis
staphylococcus aureus
biofilm formation
binding protein
genome wide
nucleic acid