siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

Marcel RaspeKatarzyna M KedzioraBram van den BroekQiaole ZhaoSander de JongJohan HerzMarieke MastopJoachim GoedhartTheodorus W J GadellaIan T YoungKees Jalink

Published in: Nature methods (2016)

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

Keyphrases

high resolution
deep learning
optical coherence tomography
convolutional neural network
single molecule
artificial intelligence
hydrogen peroxide
big data
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electronic health record
magnetic resonance
sensitive detection
cone beam