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Optimization of bioengineered heparin/heparan sulfate production for therapeutic applications.

Megan S LordMoonSun JungJohn M Whitelock
Published in: Bioengineered (2017)
Heparin has been used clinically as an anti-coagulant for more than 100 y and the major source of this therapeutic is still animal tissues. Contamination issues in some batches of heparin over 10 y ago have highlighted the need to develop alternative methods of production of this essential drug. 1 Bioengineering heparin by expressing serglycin in mammalian cells is a promising approach that was recently reported by the authors. 2 This addendum explores the approaches that the authors are taking to increase the yield of recombinantly expressed serglycin decorated with heparin/heparan sulfate focusing on cell culture and bioreactor conditions and proposes that the cell microenvironment is a key modulator of heparin biosynthesis.
Keyphrases
  • venous thromboembolism
  • growth factor
  • stem cells
  • gene expression
  • emergency department
  • single cell
  • cell therapy
  • gold nanoparticles
  • electronic health record