Veronica persica Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling.
Ki-Shuk ShimMusun ParkWon-Kyung YangHanbyeol LeeSeung-Hyung KimByung-Kil ChooSung-Wook ChaeHo-Kyoung KimTaesoo KimKi-Mo KimPublished in: Antioxidants (Basel, Switzerland) (2023)
Atopic dermatitis (AD) is chronic allergic contact dermatitis with immune dysregulation. Veronica persica has pharmacological activity that prevents asthmatic inflammation by ameliorating inflammatory cell activation. However, the potential effects of the ethanol extract of V. persica (EEVP) on AD remain elusive. This study evaluated the activity and underlying molecular pathway of EEVP in two AD models: dinitrochlorobenzene (DNCB)-induced mice and interferon (IFN)-γ/tumor necrosis factor (TNF)-α-stimulated human HaCaT keratinocytes. EEVP attenuated the DNCB-induced increase in serum immunoglobulin E and histamine levels, mast cell counts in toluidine-blue-stained dorsal skin, inflammatory cytokine (IFN-γ, interleukin [IL]-4, IL-5, and IL-13) levels in cultured splenocytes, and the mRNA expression of IL6 , IL13 , IL31 receptor , CCR-3 , and TNFα in dorsal tissue. Additionally, EEVP inhibited the IFN-γ/TNF-α-induced mRNA expression of IL6 , IL13 , and CXCL10 in HaCaT cells. Furthermore, EEVP restored the IFN-γ/TNF-α-induced downregulation of heme oxygenase (HO)-1 in HaCaT cells by inducing nuclear factor erythroid 2-related factor 2 (Nrf2) expression. A molecular docking analysis demonstrated that EEVP components have a strong affinity to the Kelch-like ECH-associated protein 1 Kelch domain. In summary, EEVP inhibits inflammatory AD by attenuating immune cell activation and inducing the Nrf2/HO-1 signaling pathway in skin keratinocytes.
Keyphrases
- oxidative stress
- atopic dermatitis
- diabetic rats
- high glucose
- induced apoptosis
- rheumatoid arthritis
- endothelial cells
- dendritic cells
- signaling pathway
- molecular docking
- immune response
- nuclear factor
- toll like receptor
- mass spectrometry
- metabolic syndrome
- neuropathic pain
- spinal cord injury
- single cell
- adipose tissue
- anti inflammatory
- mesenchymal stem cells
- endoplasmic reticulum stress
- molecular dynamics simulations
- binding protein
- cell therapy