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Identification of a tomato UDP-arabinosyltransferase for airborne volatile reception.

Koichi SugimotoEiichiro OnoTamaki InabaTakehiko TsukaharaKenya FujitaManabu HorikawaHiromi ToyonagaKohki FujikawaTsukiho OsawaShunichi HommaYoshikazu KiriiwaIppei OhmuraAtsushi MiyagawaHatsuo YamamuraMikio FujiiRika OzawaBunta WatanabeKenji MiuraHiroshi EzuraToshiyuki OhnishiJunji Takabayashi
Published in: Nature communications (2023)
Volatiles from herbivore-infested plants function as a chemical warning of future herbivory for neighboring plants. (Z)-3-Hexenol emitted from tomato plants infested by common cutworms is taken up by uninfested plants and converted to (Z)-3-hexenyl β-vicianoside (HexVic). Here we show that a wild tomato species (Solanum pennellii) shows limited HexVic accumulation compared to a domesticated tomato species (Solanum lycopersicum) after (Z)-3-hexenol exposure. Common cutworms grow better on an introgression line containing an S. pennellii chromosome 11 segment that impairs HexVic accumulation, suggesting that (Z)-3-hexenol diglycosylation is involved in the defense of tomato against herbivory. We finally reveal that HexVic accumulation is genetically associated with a uridine diphosphate-glycosyltransferase (UGT) gene cluster that harbors UGT91R1 on chromosome 11. Biochemical and transgenic analyses of UGT91R1 show that it preferentially catalyzes (Z)-3-hexenyl β-D-glucopyranoside arabinosylation to produce HexVic in planta.
Keyphrases
  • copy number
  • genome wide
  • particulate matter
  • genetic diversity
  • mass spectrometry
  • liquid chromatography
  • innate immune
  • tandem mass spectrometry