A New Escherichia coli Entry Vector Series (pIIS18) for Seamless Gene Cloning Using Type IIS Restriction Enzymes.

Hend AltaibYuka OzakiTomoya KozakaiYassien BadrIzumi NomuraTohru Suzuki

Published in: Microbiology resource announcements (2019)

A series of new Escherichia coli entry vectors (pIIS18-SapI, pIIS18-BsmBI, pIIS18-BsaI, pIIS18-BfuAI-1, and pIIS18-BfuAI-2) was constructed based on a modified pUC18 backbone, which carried newly designed multiple cloning sites, consisting of two facing type IIS enzyme cleavage sites and one blunt-end enzyme cleavage site. These vectors are useful for seamless gene cloning.

Keyphrases

escherichia coli
genome wide
copy number
dna binding
wastewater treatment
biofilm formation
gene therapy
transcription factor