Comparative study of four Yucca species by 2D-NMR and LC-MS.

The present study was conducted to compare leaf extracts of Y. gloriosa, Y. treculeana, Y. filamentosa and Y. aloifolia . Plant extracts were obtained by sequential extraction with 50% and 100% methanol using the pressurized liquid extraction method. General comparative analysis of Yucca extracts was performed by 2 D-NMR. The presence of various biologically active components in the extracts was shown, among which steroid saponins predominate. The use of LC-MS and LC-HRMS methods with electrospray ionization made it possible to conduct a comparative analysis of Yucca steroidal saponins. The 33 steroidal saponins of various structures were found. The presence of three characteristic saponins in all Yucca species was shown: yuccaloeside C, yuccaloeside E and yuccaloeside B. The results obtained can be used to control the quality of plant materials and products.