The Rapid Phenotypic Susceptibility Testing in Real-Life Experience: How the MIC Values Impact on Sepsis Fast Diagnostic Workflow.
Giuseppe MigliorisiMaddalena CalvoAntonina ColluraFrancesca Di BernardoMarianna PerezGuido ScaliaStefania StefaniPublished in: Diagnostics (Basel, Switzerland) (2023)
The MIC value definition faithfully reflects antimicrobial sensitivity, profoundly impacting the infection's clinical outcome. Our study aimed to evaluate the Accelerate Pheno TM System in defining the importance of fast phenotypic susceptibility data. A number of 270 monomicrobial samples simultaneously underwent standard procedures and fast protocols after a contemporary Gram stain. Finally, we provided Turn-around Time (TAT) and statistical evaluations. The fast technology required a medium value of 7 h to complete ID and AST profiles. Although there were some spectrum limitations, it revealed an optimal success rate in microbial identification directly from positive blood cultures. The Gram-negative AST reached a 98.9% agreement between the Accelerate Pheno™ System and the standard method. In addition, the Gram-positive AST gathered a 98.7% agreement comparing the same systems. The chance to rapidly provide precise MIC values is one of the last frontiers in clinical microbiology, especially in high-prevalence antimicrobial resistance areas.
Keyphrases
- gram negative
- antimicrobial resistance
- multidrug resistant
- electronic health record
- risk factors
- microbial community
- intensive care unit
- staphylococcus aureus
- acute kidney injury
- big data
- septic shock
- sensitive detection
- single cell
- living cells
- machine learning
- bioinformatics analysis
- loop mediated isothermal amplification