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The Effect of Multilayered Electrospun Plla Nanofibers Coated with Human Amnion Or Bladder Ecm Proteins on Epithelialization And Smooth Muscle Regeneration in The Rabbit Bladder.

Keykavoos GholamiEhsan SeyedjafariFatemeh Sadat MahdaviMehdi NaghdipoorGholamreza MesbahParisa ZahmatkeshMilad AkbarzadehmoallemkolaeiLeila Zareian BaghdadabadShiv Kumar PandianKirolos N MeilikaSeyed Mohammad Kazem Aghamir
Published in: Macromolecular bioscience (2023)
Nanofibrous scaffolds have attracted much attention in bladder reconstruction approaches due to their excellent mechanical properties. In addition, their biological properties can be improved by combination with biological materials. Taking into account the advantages of nanofibrous scaffolds and decellularized extracellular matrix (dECM) in tissue engineering, scaffolds of poly-L-lactic acid (PLLA) coated with decellularized human amnion membrane (hAM) or sheep bladder (SB)-derived ECM proteins are developed (amECM-coated PLLA and sbECM-coated PLLA, respectively). The bladder regenerative potential of modified electrospun PLLA scaffolds is investigated in rabbits. The presence of ECM proteins is confirmed on the nanofibers' surface. Coating the surface of the PLLA nanofibers improves cell adhesion and proliferation. Histological and immunohistochemical evaluations show that rabbits subjected to cystoplasty with a multilayered PLLA scaffold show de novo formation and maturation of the multilayered urothelial layer. However, smooth muscle bundles (myosin heavy chain [MHC] and α-smooth muscle actin [α-SMA] positive) are detected only in ECM-coated PLLA groups. All groups show no evidence of a diverticulumor fistula in the urinary bladder. These results suggest that the biofunctionalization of electrospun PLLA nanofibers with ECM proteins could be a promising option for bladder tissue engineering. Furthermore, hAM can also replace animal-sourced ECM proteins in bladder tissue regeneration approaches. This article is protected by copyright. All rights reserved.
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