The interaction between the Dbf4 ortholog Chiffon and Gcn5 is conserved in Dipteran insect species.
Smitha GeorgeHannah R BlumEliana F Torres-ZeladaGrace N EstepYoussef A HegazyGina M SpeerVikki M WeakePublished in: Insect molecular biology (2022)
Chiffon is the sole Drosophila ortholog of Dbf4, the regulatory subunit for the cell-cycle kinase Cdc7 that initiates DNA replication. In Drosophila, the chiffon gene encodes two polypeptides with independent activities. Chiffon-A contains the conserved Dbf4 motifs and interacts with Cdc7 to form the Dbf4-dependent Kinase (DDK) complex, which is essential for a specialized form of DNA replication. In contrast, Chiffon-B binds the histone acetyltransferase Gcn5 to form the Chiffon histone acetyltransferase (CHAT) complex, which is necessary for histone H3 acetylation and viability. Previous studies have shown that the Chiffon-B region is only present within insects. However, it was unclear how widely the interaction between Chiffon-B and Gcn5 was conserved among insect species. To examine this, we performed yeast two-hybrid assays using Chiffon-B and Gcn5 from a variety of insect species and found that Chiffon-B and Gcn5 interact in Diptera species such as Australian sheep blowfly and yellow fever mosquito. Protein domain analysis identified that Chiffon-B has features of acidic transcriptional activators such as Gal4 or VP16. We propose that the CHAT complex plays a critical role in a biological process that is unique to Dipterans and could therefore be a potential target for pest control strategies.
Keyphrases
- genome wide identification
- transcription factor
- cell cycle
- aedes aegypti
- cell proliferation
- dna methylation
- protein kinase
- magnetic resonance
- high throughput
- gene expression
- tyrosine kinase
- palliative care
- oxidative stress
- magnetic resonance imaging
- computed tomography
- risk assessment
- climate change
- small molecule
- human health
- protein protein
- contrast enhanced
- single cell
- case control