Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.
Shutoku MatsuyamaNaganori NaoKazuya ShiratoMiyuki KawaseShinji SaitoIkuyo TakayamaNoriyo NagataTsuyoshi SekizukaHiroshi KatohFumihiro KatoMasafumi SakataMaino TaharaSatoshi KutsunaNorio OhmagariMakoto KurodaTadaki SuzukiTsutomu KageyamaMakoto TakedaPublished in: Proceedings of the National Academy of Sciences of the United States of America (2020)
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.