BK Polyomavirus Diversity After Hematopoietic Stem Cell Transplantation.
Elizabeth A OdegardHeidi L MeedsSteven B KleiboekerAssem ZiadyAnthony SabulskiSonata JodeleAlix E SeifStella M DaviesBenjamin L LaskinJason Tory BlackardPublished in: The Journal of infectious diseases (2023)
BK polyomavirus (BKPyV) infection is common after hematopoietic stem cell transplantation (HSCT) and is associated with the development of hemorrhagic cystitis (HC). The role that BKPyV plays in the pathogenesis of HC is not well characterized. We investigated the impact of BKPyV diversity on the development of HC using a previously established cohort of pediatric HSCT patients. There were 147 urine samples with quantifiable BKPyV at month 1 after HSCT; 137 (93.2%) were amplified using our in-house polymerase chain reaction approach and sent for next-generation sequencing. Subtype Ia was most frequent (61.3%), followed by subtype Ib1 (31.4%). The median viral load of subtype Ia samples was higher than for subtype Ib1 at month 1. Across the protein coding regions, APOBEC-induced mutations and signature patterns associated with HC were identified. This is the largest sequencing study of a single cohort of HSCT patients, providing a vast resource of sequence data for future analyses.
Keyphrases
- end stage renal disease
- newly diagnosed
- ejection fraction
- chronic kidney disease
- acute myeloid leukemia
- prognostic factors
- hematopoietic stem cell
- dna methylation
- patient reported outcomes
- gene expression
- peritoneal dialysis
- high glucose
- mass spectrometry
- single cell
- atomic force microscopy
- endothelial cells
- current status