Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the γ-Ray Irradiation Technique.
Young-Pil YunJae Yong LeeWon Jae JeongKyeongsoon ParkHak-Jun KimJae Jun SongSung Eun KimHae-Ryong SongPublished in: BioMed research international (2015)
The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the γ-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the γ-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP-2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and the mRNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells.
Keyphrases
- mesenchymal stem cells
- induced apoptosis
- bone regeneration
- high resolution
- cell cycle arrest
- oxidative stress
- computed tomography
- magnetic resonance imaging
- bone marrow
- magnetic resonance
- endothelial cells
- gene expression
- genome wide
- risk assessment
- cell proliferation
- transcription factor
- high glucose
- single molecule
- radiation induced
- pi k akt
- diabetic rats
- contrast enhanced
- liquid chromatography