UPLC-QTOF-MS-guided isolation of anti-COPD ginsenosides from wild ginseng.
Hailin ZhuJunli LiuHongqiang LinYing ZhangNa YangBaisong ZhouZhongyao WangAlan Chen-Yu HsuJinping LiuPingya LiPublished in: RSC advances (2019)
Four previously undescribed ginsenosides, along with five known analogues were isolated from wild ginseng by a UPLC-QTOF-MS-guided fractionation procedure. Their structures were elucidated on the basis of spectroscopic and spectrometric data (1D and 2D NMR, HR-ESI-MS). The isolated compounds could significantly inhibit the cigarette smoke extract (CSE)-induced inflammatory reaction in A549 cells. The HDAC2 pathway might be involved in the protective effect against the CSE-mediated inflammatory response in A549 cells.
Keyphrases
- ms ms
- induced apoptosis
- mass spectrometry
- inflammatory response
- multiple sclerosis
- cell cycle arrest
- oxidative stress
- molecular docking
- chronic obstructive pulmonary disease
- high resolution
- magnetic resonance
- signaling pathway
- immune response
- high performance liquid chromatography
- liquid chromatography
- minimally invasive
- big data
- diabetic rats
- lipopolysaccharide induced
- machine learning
- deep learning
- molecular dynamics simulations
- air pollution
- solid phase extraction