AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosis.
R StöhrN DeckersLeon J SchurgersN MarxChris P M ReutelingspergerPublished in: Scientific reports (2018)
Efferocytosis, the clearing of dead or dying cells from living tissues, is a highly programmed, vital process to maintain the healthy functioning of every organism. Disorders of efferocytosis have been linked to several chronic diseases including atherosclerosis and auto-immune diseases. To date several different assays to determine phagocytosis, using microscopy or FACS analysis with labelled targets, have been developed. However, many of these are unable to differentiate between cells that have truly been phagocytosed and those still present on the surface of the macrophages hindering exact assessment of efferocytotic capacity. We herein describe AnxA5-pHrodo and its negative control M1234-pHrodo as new molecular probes to measure in vitro as well as ex-vivo efferocytotic capacity.
Keyphrases
- single molecule
- living cells
- induced apoptosis
- high throughput
- cell cycle arrest
- palliative care
- small molecule
- high resolution
- cardiovascular disease
- gene expression
- optical coherence tomography
- quantum dots
- density functional theory
- signaling pathway
- type diabetes
- oxidative stress
- mass spectrometry
- fluorescent probe
- cell death
- pi k akt