Quantifying the phase separation property of chromatin-associated proteins under physiological conditions using an anti-1,6-hexanediol index.

Minglei ShiKaiqiang YouTaoyu ChenChao HouZhengyu LiangMingwei LiuJifeng WangTaotao WeiJun QinYang ChenMichael Q ZhangTingting Li

Published in: Genome biology (2021)

Our work provides a global quantitative measurement of LLPS properties of chromatin-associated proteins and higher-order chromatin structure. Hi-MS and AICAP data provide an experimental tool and quantitative resources valuable for future studies of biomolecular condensates.

Keyphrases

dna damage
gene expression
transcription factor
genome wide
high resolution
mass spectrometry
ms ms
electronic health record
dna methylation
oxidative stress