Antimicrobial Photodynamic Inactivation Mediated by Rose Bengal and Erythrosine Is Effective in the Control of Food-Related Bacteria in Planktonic and Biofilm States.
Alex Fiori SilvaAnabela BorgesCamila Fabiano de Freitas MarinNoboru HiokaJane Martha Graton MikchaMariana SousaPublished in: Molecules (Basel, Switzerland) (2018)
The thermal and chemical-based methods applied for microbial control in the food industry are not always environmentally friendly and may change the nutritional and organoleptic characteristics of the final products. Moreover, the efficacy of sanitizing agents may be reduced when microbial cells are enclosed in biofilms. The objective of this study was to investigate the effect of photodynamic inactivation, using two xanthene dyes (rose bengal and erythrosine) as photosensitizing agents and green LED as a light source, against Staphylococcus aureus, Listeria innocua, Enterococcus hirae and Escherichia coli in both planktonic and biofilm states. Both photosensitizing agents were able to control planktonic cells of all bacteria tested. The treatments altered the physicochemical properties of cells surface and also induced potassium leakage, indicating damage of cell membranes. Although higher concentrations of the photosensitizing agents (ranging from 0.01 to 50.0 μmol/L) were needed to be applied, the culturability of biofilm cells was reduced to undetectable levels. This finding was confirmed by the live/dead staining, where propidium iodide-labeled bacteria numbers reached up to 100%. The overall results demonstrated that photoinactivation by rose bengal and erythrosine may be a powerful candidate for the control of planktonic cells and biofilms in the food sector.
Keyphrases
- induced apoptosis
- staphylococcus aureus
- cell cycle arrest
- escherichia coli
- pseudomonas aeruginosa
- candida albicans
- biofilm formation
- endoplasmic reticulum stress
- cell death
- stem cells
- signaling pathway
- microbial community
- multidrug resistant
- cell proliferation
- cancer therapy
- klebsiella pneumoniae
- pi k akt
- diabetic rats
- aqueous solution