A novel mitochondrial-targeting fluorescent probe based on 1,4-dihydropyridine to visualize and monitor the viscosity of live cells and mice in vivo.
Longqi XueJialin LvRonghang Linull Xinyu WangYapeng LiJian-Shi DuShaolong QiQingbiao YangYaming ShanYaoxian LiPublished in: Analytical methods : advancing methods and applications (2021)
Cell viscosity is related to some diseases, such as diabetes, atherosclerosis, and Alzheimer's disease. These diseases can cause abnormal viscosity of the cell mitochondrial matrix. 1,4-Dihydropyridine (DHP) is an important organic compound with biological activity and is widely used in drug research. However, there are few studies on its optical properties, especially in the design of viscous fluorescent probes. In this study, a fluorescent probe for viscosity detection using 1,4-dihydropyridine as the fluorophore and indole iodide salt as the recognition group was designed and synthesized. The probe has the advantages of a deep-red emission, low cytotoxicity, good biocompatibility and excellent anti-interference ability. In addition, the probe also has the ability to target mitochondria and has been successfully applied to the detection of the viscosity response of HeLa cells and living mice, and has good clinical application potential.
Keyphrases
- living cells
- fluorescent probe
- cell cycle arrest
- induced apoptosis
- oxidative stress
- cell death
- single molecule
- single cell
- cardiovascular disease
- cell therapy
- type diabetes
- loop mediated isothermal amplification
- high fat diet induced
- quantum dots
- real time pcr
- small molecule
- signaling pathway
- skeletal muscle
- climate change
- glycemic control
- bone marrow
- water soluble
- risk assessment
- fluorescence imaging
- tissue engineering
- cell proliferation
- drug delivery
- endoplasmic reticulum
- solid state