Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification.
Madhusudan B KulkarniSanket GoelPublished in: BioTechniques (2023)
The recent cases of COVID-19 have brought the prospect of and requirement for point-of-care diagnostic devices into the limelight. Despite all the advances in point-of-care devices, there is still a huge requirement for a rapid, accurate, easy-to-use, low-cost, field-deployable and miniaturized PCR assay device to amplify and detect genetic material. This work aims to develop an Internet-of-Things automated, integrated, miniaturized and cost-effective microfluidic continuous flow-based PCR device capable of on-site detection. As a proof of application, the 594-bp GAPDH gene was successfully amplified and detected on a single system. The presented mini thermal platform with an integrated microfluidic device has the potential to be used for the detection of several infectious diseases.
Keyphrases
- high throughput
- label free
- loop mediated isothermal amplification
- real time pcr
- low cost
- infectious diseases
- single cell
- circulating tumor cells
- copy number
- coronavirus disease
- sars cov
- genome wide
- machine learning
- single molecule
- deep learning
- health information
- sensitive detection
- social media
- dna methylation
- healthcare
- nucleic acid
- circulating tumor
- high resolution