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Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Kerstin RennerTobias SchwittaySophia ChaabaneJohanna GottschlingChristine MüllerCharlotte TiefenböckJan-Niklas SalewskiFrederike WinterSimone BuchtlerSaidou BalamMaximilian Valentin MalfertheinerMatthias LubnowDirk LunzBernhard GrafFlorian HitzenbichlerFrank HansesHendrik PoeckMarina KreutzEvelyn OrsóRalph BurkhardtTanja NiedermairChristoph BrochhausenAndré GessnerBernd SalzbergerMatthias Mack
Published in: Nature communications (2021)
Coronavirus disease 2019 (COVID-19) can lead to pneumonia and hyperinflammation. Here we show a sensitive method to measure polyclonal T cell activation by downstream effects on responder cells like basophils, plasmacytoid dendritic cells, monocytes and neutrophils in whole blood. We report a clear T cell hyporeactivity in hospitalized COVID-19 patients that is pronounced in ventilated patients, associated with prolonged virus persistence and reversible with clinical recovery. COVID-19-induced T cell hyporeactivity is T cell extrinsic and caused by plasma components, independent of occasional immunosuppressive medication of the patients. Monocytes respond stronger in males than females and IL-2 partially restores T cell activation. Downstream markers of T cell hyporeactivity are also visible in fresh blood samples of ventilated patients. Based on our data we developed a score to predict fatal outcomes and identify patients that may benefit from strategies to overcome T cell hyporeactivity.
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