Proximicins F and G and Diproximicin A: Aminofurans from the Marine-Derived Verrucosispora sp. SCSIO 40062 by Overexpression of PPtase Genes.
Chunyan FangQingbo ZhangYiguang ZhuLiping ZhangWenjun ZhangLiang MaHaibo ZhangChangsheng ZhangPublished in: Journal of natural products (2020)
Overexpression of phosphopantetheinyl transferase (PPtase)-encoding genes sfp and svp in the marine-derived Verrucosispora sp. SCSIO 40062 led to the production of two new aminofuran monomers, proximicin F (1) and proximicin G (3) and a new dimer diproximicin A (2), along with two known compounds, proximicins B (4) and C (5). Their structures were unambiguously elucidated on the basis of detailed NMR spectroscopic analysis and high-resolution electrospray ionization mass spectrometry (HRESIMS) data. Proximicin B (4) showed moderate antibacterial activities against Staphylococcus aureus, methicillin-resistant S. aureus, and Bacillus subtilis.
Keyphrases
- high resolution
- staphylococcus aureus
- bacillus subtilis
- mass spectrometry
- genome wide
- cell proliferation
- genome wide identification
- transcription factor
- bioinformatics analysis
- methicillin resistant staphylococcus aureus
- liquid chromatography
- molecular docking
- electronic health record
- magnetic resonance
- tandem mass spectrometry
- high performance liquid chromatography
- escherichia coli
- data analysis
- silver nanoparticles
- simultaneous determination