Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia.
Narendra Kumar SharmaAlexandre Keiji TashimaMilena Karina Colo BrunialtiÉden Ramalho FerreiraRicardo Jose Soares TorquatoRenato Arruda MortaraFlavia Ribeiro MachadoMurillo AssuncaoOtelo RigatoReinaldo SalomãoPublished in: Scientific reports (2017)
Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age- and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis.
Keyphrases
- end stage renal disease
- gene expression
- newly diagnosed
- acute kidney injury
- chronic kidney disease
- prognostic factors
- peritoneal dialysis
- intensive care unit
- immune response
- dna methylation
- physical activity
- community acquired pneumonia
- single cell
- metabolic syndrome
- mental health
- oxidative stress
- long non coding rna
- hydrogen peroxide
- endoplasmic reticulum stress
- copy number
- dendritic cells
- induced apoptosis
- cardiovascular events
- genome wide
- rna seq
- septic shock
- glycemic control
- aqueous solution