A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum.

Sarah JurischkaAstrid BidaDoris Dohmen-OlmaBritta KleineJanko PotzkeiStephan BinderGeorg SchaumannPatrick J BakkesRoland Freudl

Published in: Microbial cell factories (2020)

We have succeeded in the construction of a C. glutamicum biosensor strain that allows for the monitoring of Sec-dependent secretion of heterologous proteins in a dose-dependent manner, independent of a direct assay for the desired target protein.

Keyphrases

sensitive detection
gold nanoparticles
protein protein
quantum dots
label free
high throughput
binding protein
small molecule