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Optimizing the Toehold Strategy of On-Chip Nucleic Acid Hybridization Probe for the Discrimination of Single Nucleotide Polymorphism.

Xiang ZhouDongbao YaoMiao HeShiyan XiaoHaojun Liang
Published in: Langmuir : the ACS journal of surfaces and colloids (2018)
The synthetic DNA hybridization probe has proved its importance in biology and biotechnology. In this study, taking advantage of a novel analytical technique called dual polarization interferometry (DPI), the influence of the toehold strategy of on-chip DNA hybridization probe on the discrimination of single nucleotide polymorphism (SNP) was investigated. Through adjusting the toehold length, the toehold strategies of on-chip toehold exchange probe were thoroughly optimized. For the "6/5" probe, an optimal discrimination factor of 78% against the spurious target was achieved. Moreover, the ability of the on-chip probe in SNP discrimination was significantly enhanced compared to its pure solution counterpart. This simple and rapid detection method for SNP discrimination based on the on-chip toehold exchange probe will show great potential in disease diagnosis.
Keyphrases
  • nucleic acid
  • living cells
  • quantum dots
  • single molecule
  • high throughput
  • circulating tumor cells
  • genome wide
  • circulating tumor
  • cell free
  • label free
  • genetic diversity
  • liquid chromatography