Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes.
Zhongying WangTatyana GurloAleksey V MatveyenkoDavid ElashoffPeiyu WangMadeline RosenbergerJason A JungeRaymond C StevensKate L WhiteScott E FraserPeter J ButlerPublished in: Communications biology (2021)
Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.
Keyphrases
- induced apoptosis
- type diabetes
- cell cycle arrest
- cardiovascular disease
- endoplasmic reticulum stress
- public health
- healthcare
- cell death
- stem cells
- mental health
- blood pressure
- climate change
- cell proliferation
- glycemic control
- single cell
- adipose tissue
- drug discovery
- photodynamic therapy
- mesenchymal stem cells
- risk assessment
- protein kinase
- cell therapy
- insulin resistance
- room temperature
- social media
- stress induced