Development of a new tandem mass spectrometry method for urine and amniotic fluid screening of oligosaccharidoses.
Monique PiraudMagali PettazzoniLouise MenegautCatherine CaillaudYann NadjarChristine Vianey-SabanRoseline FroissartPublished in: Rapid communications in mass spectrometry : RCM (2018)
The method is quick and easy to run, with an LC analysis time of 13 min per sample. The quantitative validation could not be obtained in the absence of a specific standard and of a labelled internal standard for each compound. Even if this LC/MS/MS method is only qualitative, it is very specific and much more sensitive than TLC. It allows the urinary screening of oligosaccharidoses, even mild or late-onset forms, and the screening of antenatal forms in amniotic fluid. Copyright © 2017 John Wiley & Sons, Ltd.
Keyphrases
- late onset
- tandem mass spectrometry
- simultaneous determination
- liquid chromatography
- ultra high performance liquid chromatography
- high performance liquid chromatography
- early onset
- high resolution
- gas chromatography
- pregnant women
- mass spectrometry
- solid phase extraction
- preterm birth
- high resolution mass spectrometry
- mesenchymal stem cells