RN7SL1 may be translated under oncogenic conditions.
Tomoaki HaraSikun MengYoshiko TsujiYasuko AraoYoshiko SaitoHiromichi SatoDaisuke MotookaShizuka UchidaHideshi IshiiPublished in: Proceedings of the National Academy of Sciences of the United States of America (2024)
RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.