Chemical binding of horseradish peroxidase enzyme with poly beta-cyclodextrin and its application as molecularly imprinted polymer for the monitoring of H2 O2 in human plasma samples.
Sanam SardaremelliMohammad HasanzadehHabib RazmiPublished in: Journal of molecular recognition : JMR (2021)
In this study, a selective and sensitive molecular imprinting-based electrochemical sensors, for horseradish peroxidase (HRP) entrapment was fabricated using electro polymerization of ß-Cyclodextrin (ß-CD) on the surface of glassy carbon electrode. Poly beta-cyclodextrin P(ß-CD) provide efficient surface area for self-immobilization of HRP as well as improve imprinting efficiency. The proposed imprinted biosensor successfully utilized for detection of HRP with excellent analytical results which linear range is 0.1 mg/mL to 10 ng/mL with LOD of 2.23 ng/mL. Furthermore, electrocatalytical activity of the prepared biosensor toward the reduction of hydrogen peroxide was investigated in the ranges of 1 to 15 μM with a detection limit of 0.4 μM by using chronoamperometry technique. The developed biosensor was used for the detection of hydrogen peroxide in unprocessed human plasma sample.
Keyphrases
- hydrogen peroxide
- label free
- molecularly imprinted
- nitric oxide
- gold nanoparticles
- loop mediated isothermal amplification
- sensitive detection
- ionic liquid
- real time pcr
- quantum dots
- solid phase extraction
- capillary electrophoresis
- single molecule
- nk cells
- binding protein
- atomic force microscopy
- high resolution
- carbon nanotubes