Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic.

Zhichao ChenFeng XiongAimin Yu Guosong Lai

Published in: Chemical communications (Cambridge, England) (2019)

A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for "off-on" signal transduction. The special hairpin structure of the aptamer-kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.

Keyphrases

label free
gold nanoparticles
hydrogen peroxide
living cells
sensitive detection
fluorescent probe
cell free
single molecule
nucleic acid
mass spectrometry
high resolution
simultaneous determination
solid phase extraction