Multidimensional Conductive Nanofilm-Based Flexible Aptasensor for Ultrasensitive and Selective HBsAg Detection.
Kyung Hee ChoDong Hoon ShinJungkyun OhJi Hyun AnJun Seop LeeJyongsik JangPublished in: ACS applied materials & interfaces (2018)
Hepatitis B virus (HBV) infection is a major worldwide health issue causing serious liver diseases, including liver cirrhosis and hepatocellular carcinoma. Monitoring the serum hepatitis B surface antigen (HBsAg) level is pivotal to the diagnosis of HBV infection. In this study, we describe multidimensional conductive nanofilm (MCNF)-based field-effect transistor (FET) aptasensor for HBsAg detection. The MCNF, composed of vertically oriented carboxylic polypyrrole nanowires (CPPyNW) and graphene layer, is formed using electropolymerization of pyrrole on the graphene surface and following acid treatment. The amine-functionalized HBsAg-binding aptamers are then immobilized on the CPPyNW surface through covalent bonding formation (i.e., amide group). The prepared aptasensor presents highly sensitive to HBsAg as low as 10 aM among interfering biomolecules with various deformations. Moreover, the MCNF-based aptasensor has great potential for practical application in the noninvasive real-time diagnosis because of its improved sensing ability to the human serum and artificial saliva.
Keyphrases
- hepatitis b virus
- label free
- quantum dots
- sensitive detection
- reduced graphene oxide
- liver failure
- public health
- healthcare
- room temperature
- molecularly imprinted
- energy transfer
- carbon nanotubes
- gold nanoparticles
- human health
- mental health
- mass spectrometry
- risk assessment
- psychometric properties
- ionic liquid
- tissue engineering
- health promotion
- fluorescent probe
- solid phase extraction
- living cells