Development of a Pre-assembled Through-Bond Energy Transfer (TBET) Fluorescent Probe for Ratiometric Sensing of Anticancer Platinum(ll) Complexes.
Jun Xiang OngWee Han AngPublished in: Chemistry, an Asian journal (2020)
Fluorescence microscopy has emerged as an attractive technique to probe the intracellular processing of Pt-based anticancer compounds. Herein, we reported the first through-bond energy transfer (TBET) fluorescent probe NPR1 designed for sensitive detection and quantitation of PtII complexes. The novel TBET probe was successfully applied for ratiometric fluorescence imaging of anticancer PtII complexes such as cisplatin and JM118 in cells. Capitalizing on the ability of the probe to discriminate between PtII complexes and their PtIV derivatives, the probe was further applied to study the activation of PtIV prodrug complexes that are known to release active PtII species after intracellular reduction.
Keyphrases
- living cells
- fluorescent probe
- energy transfer
- quantum dots
- sensitive detection
- single molecule
- fluorescence imaging
- induced apoptosis
- high resolution
- nitric oxide
- photodynamic therapy
- mass spectrometry
- high throughput
- ms ms
- high speed
- oxidative stress
- hydrogen peroxide
- liquid chromatography tandem mass spectrometry
- drug delivery
- transition metal