Investigation of bone regeneration efficacy of new bovine bone minerals in a canine mandibular critical defect model.

This study aimed to investigate the bone regeneration effect of bovine hydroxyapatite-processed biomaterials Bone-XB and S1-XB in a beagle mandibular defect model. A total of four saddle-type critical sizes (15 mm × 10 mm) bone defects were created in each dog: two defects in the left mandible and two defects in the right mandible. The defect control (DC) group was kept unfilled, and the other three defects were filled with three different biomaterials as follows: positive control Bio-Oss ® (Bio-Oss group), Bone-XB (XB group), and S1-XB (S1-XB group). Bone regeneration was evaluated by radiography, micro-computed tomography, and histological analysis. we revealed that Bone-XB and S1-XB significantly increased newly formed bone, defect filling percentage, and bone healing score compared to the DC group, which was confirmed by bone microstructure augmentation (bone volume/total volume, trabecular number, and trabecular thickness). Interestingly, no significant differences were observed between the Bone-XB, S1-XB, and Bio-Oss groups. We suggest that Bone-XB or S1-XB stimulates bone regeneration demonstrated by the increase in newly formed bone and bone microstructure, thereby improving bone defect filling, which was equivalent to the Bio-Oss ® . Therefore, bovine hydroxyapatite-processed Bone-XB or S1-XB could be considered effective biomaterials for correcting critical-size bone defects or fractures. This article is protected by copyright. All rights reserved.